Composite

Part:BBa_M36587

Designed by: Aris Kare   Group: Stanford BIOE44 - S11   (2015-10-24)


Making acetone from acetoacetate using ADC

This part begins with a promoter, then the ADC coding sequence, and finally, a termination sequence from T1. This composite part aims to produce acetone from acetoacetate using the gene sequence of ADC. ADC, or acetoacetate decarboxylase, is able to convert the byproducts acetoacetate and butyryl-CoA (made by the gene sequences ctfA and ctfB) into acetate, the final product in this complex mechanism. This can be used in projects that are aiming to convert acetoacetate especially into acetone.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
    Illegal PstI site found at 721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
    Illegal PstI site found at 721
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
    Illegal PstI site found at 721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
    Illegal PstI site found at 721
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None